SCSAP SPEAKER SPOTLIGHT
Evan Macosko, M.D., Ph.D.
Associate Professor Psychiatry, Massachusetts General Hospital, Institute Member, Broad Institute
Bio: Evan Macosko is an institute member at the Broad Institute of MIT and Harvard, where he directs a lab in the Stanley Center for Psychiatric Research. He is also an associate professor of psychiatry and neurobiology at Harvard Medical School, and attending psychiatrist at Massachusetts General Hospital. He serves as the Co-PI for the BICAN Brain Cell Variation grant. His lab develops new genomics technologies and deploys them to understand pathogenic mechanisms of brain diseases.
Abstract: The advent of next-generation sequencing ushered in a ten-year period of exuberant technology development, enabling the quantification of gene expression and epigenetic features within individual cells, and within intact tissue sections. In this seminar, I will outline our technological contributions, beginning with the development of Drop-seq, a method for high-throughput single-cell analysis, followed by the development of Slide-seq, a technique for measuring genome-wide expression at 10-micron spatial resolution. Using a combination of these techniques, we recently constructed a comprehensive cell type atlas of the adult mouse brain, positioning cell types within individual brain structures. I will discuss the major findings from this dataset, including emerging principles of neurotransmission, and the localization of disease gene signatures to specific cell types. Finally, I will introduce a new spatial technology, Slide-tags, that unifies single-cell and spatial genomics into a single, highly scalable assay.